Plant Transcription Factor Database
Previous version: v3.0
Solanum lycopersicum
HSF Family
Species TF ID Description
Solyc02g072000.2.1HSF family protein
Solyc02g072060.1.1HSF family protein
Solyc02g078340.2.1HSF family protein
Solyc02g079180.1.1HSF family protein
Solyc02g090820.2.1HSF family protein
Solyc03g006000.2.1HSF family protein
Solyc03g026020.2.1HSF family protein
Solyc03g097120.2.1HSF family protein
Solyc04g016000.2.1HSF family protein
Solyc04g078770.2.1HSF family protein
Solyc06g053960.2.1HSF family protein
Solyc06g072750.2.1HSF family protein
Solyc07g040680.2.1HSF family protein
Solyc07g055710.2.1HSF family protein
Solyc08g005170.2.1HSF family protein
Solyc08g062960.2.1HSF family protein
Solyc08g076590.2.1HSF family protein
Solyc08g080540.2.1HSF family protein
Solyc09g009100.2.1HSF family protein
Solyc09g059520.2.1HSF family protein
Solyc09g065660.2.1HSF family protein
Solyc09g082670.2.1HSF family protein
Solyc10g079380.1.1HSF family protein
Solyc11g064990.1.1HSF family protein
Solyc12g007070.1.1HSF family protein
Solyc12g098520.1.1HSF family protein
HSF Family Introduction

Heat stress transcription factors (Hsfs) are the major regulators of the plant heat stress (hs) response. Sequencing of the Arabidopsis genome revealed the existence of 21 open-reading frames (ORFs) encoding putative Hsfs assigned to classes A-C. Here we present results of a functional genomics approach to the Arabidopsis Hsf family focused on the analysis of their C-terminal domains (CTDs) harboring conserved modules for their function as transcription factors and their intracellular localization. Using reporter assays in tobacco protoplasts and yeast as well as glutathione-S-transferase (GST) pull-down assays, we demonstrate that short peptide motifs enriched with aromatic and large hydrophobic amino acid (aa) residues embedded in an acidic surrounding (AHA motifs) are essential for transcriptional activity of class A Hsfs. In contrast to this, class B and C Hsfs lack AHA motifs and have no activator function on their own. We also provide evidence for the function of a leucine (Leu)-rich region centered around a conserved QMGΦL motif at the very C-terminus as a nuclear export signal (NES) of class A Hsfs. Sequence comparison indicates that the combination of a C-terminal AHA motif with the consensus sequence FWxxF/L,F/I/L as well as the adjacent NES represents a signature domain for plant class A Hsfs, which allowed to identify more than 60 new Hsfs from the expressed sequence tag (EST) database.

Kotak S, Port M, Ganguli A, Bicker F, von Koskull-Doring P.
Characterization of C-terminal domains of Arabidopsis heat stress transcription factors (Hsfs) and identification of a new signature combination of plant class A Hsfs with AHA and NES motifs essential for activator function and intracellular localization.
Plant J, 2004. 39(1): p. 98-112.
PMID: 15200645